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پرایمر الیگونوکلئوتیدی
Two pairs of oligonucleotide primers are used in the PCR; one set of flanking primers anneals to the ends of the target gene (often a cloned gene), and the other set consists of overlapping, internal primers that carry the mutation.
Random Mutagenesis with Degenerate Oligonucleotide Primers
In this way, the oligonucleotide primer prepa ration contains a heterogeneous set of DNA sequences that will generate a series of mutations that are clustered in a defined portion of the target gene.
Figure 3.51 Chemical synthesis of oligonucleotide primers with any of the four nucleotides at defined positions.
Using degenerate oligonucleotide primers, it was possible to intro duce a range of different mutations into the three CDRs of the variable region of an antibody heavychain gene (Fig. 3.58).
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